Research Paper

PNA HyBeacons for analysis of human mutations related to statin-induced myopathy

Volume 2, Issue 3   July - December 2011
Pages 79 - 89
Keywords: fluorescence melting, genetic analysis, HyBeacon, PNA, SNPs, statin-induced myopathy
Authors: Nittaya Gale, Petr Kocalka, Charlotte Mardle and Tom Brown

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Aminoalkyl and alkyne-tagged PNA HyBeacons have been synthesized, labeled with fluorescein via conventional amide bond or triazole formation (click chemistry) and used to detect single nucleotide polymorphisms (SNPs) implicated in statin-induced myopathy. The PNA HyBeacons gave much better mismatch/mutant discrimination than conventional DNA HyBeacons but smaller fluorescence changes on melting.

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